All REDFIN features at a glance
General
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| EXTENDING | Extend any completed analyses with new gels | Access from start page |
| RE-ANALYZING | Redo any completed analysis at no extra cost | Access from start page |
| ACCESS ANYWHERE | Access analysis results on any computer | Access from start page |
| AUTOMATIC BACKUP | REDFIN automatically backs up project data | Happens automatically |
| ONLINE STORAGE | Project data are securely stored online | Happens automatically |
| SHARING | Share analysis results interactively with colleagues | Start page: “share” icon in actions column |
| SUPPORT | Access to REDFIN experts and extensive online support material | Start page: Click “help” in top menu |
Software Installation & Updates
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| INSTALLATION | Easy one-click installation | From ludesi.com/download or expressionproteomics.com/redfin |
| SOFTWARE UPDATES | Automatic and free software updates | REDFIN updates when started |
| MULTIPLE INSTALLATIONS | Unlimited installations on different computers | Install REDFIN at work or at home, or in a different office |
Create New Analysis
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| IMAGE QUALITY CHECK | Automatic image quality check | “Create new analysis” wizard |
| IMAGE FORMATS | Supports .TIF and .GEL image formats | “Create new analysis” wizard |
| IMAGE STAINING | Supports all types of staining incl. DIGE | “Create new analysis” wizard |
| IMAGE EDITOR | Integrated image editor to rotate flip and invert images | “Create new analysis” wizard |
| TECHNICAL REPLICATES | Defining of technical replicates | “Create new analysis” wizard |
| REGION OF INTEREST | Define region of interest for analysis | “Create new analysis” wizard |
| STATISTICAL COMPARISONS | Unlimited number of groups and comparisons | “Create new analysis” wizard |
Warping (Solo analysis only)
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| AUTOMATIC WARPING | Automatic warping wizard for batch warping | “Create new analysis” wizard |
| ALIGNMENT TREND | Visual correlation trend for indication of warp improvement | “Create new analysis” wizard |
| WARP ANIMATION | Alpha blending between warping pair for visual warping assistance | “Create new analysis” wizard |
| AUTOMATIC ANCHOR DELETION | One-click removal of all automatically placed warp anchors | “Create new analysis” wizard |
| MANUAL ANCHOR EDITING | Manual adding or removal of individual warp anchors | “Create new analysis” wizard |
| ANCHOR COUNT | Automatic warp anchor counter for every warped gel | “Create new analysis” wizard |
Spot Detection and Quantification (Solo analysis only)
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| SENSITIVITY SLIDER | Spot number slider to adjust detection sensitivity | “Create new analysis” wizard |
| GLOBAL SPOT EDITING | Addition/removal of individual spots on the composite gel for fine-tuning | “Create new analysis” wizard |
| BORDER SIZE SLIDER | Border fit slider to adjust global spot border size | “Create new analysis” wizard |
| NORMALIZATION & BACKGROUND SUBTRACTION | Automatic normalization and background subtraction | “Create new analysis” wizard |
| NOISE & SPECKLES FILTER | Automatic removal of noise and speckles during spot detection | "Create new analysis" wizard (During spot detection) |
Results Visualization And Editing
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| LIST CENTRIC VIEW | View spots in a list | Results view - Radio button in toolbar |
| GEL CENTRIC VIEW | View spots in a gel image | Results view - Radio button in toolbar |
| SPOT CENTRIC VIEW | View individual spots in detail | Results view - Radio button in toolbar |
| EDIT GROUPS | Edit groups and comparisons post-analysis | Results view - Click on the “manage comparisons” tab |
| SPREAD PLOT | Spread plot of individual data points | Results view - In spot centric view |
| BAR PLOT | Bar graph of individual data points | Results view - In spot centric view |
| CONTRASTS | Contrast enhancement | Results view - Checkbox in toolbar |
| BRIGHTNESS | Brightness controls | Results view - Slider in toolbar |
| COLOR | Color mode | Results view - Checkbox in toolbar |
| ZOOMING | Zooming | Results view - Slider in gel- and spot view |
| 3D VIEW | 3D Visualization | Results view - hover over images in spot view to click on the 3D icon |
| NEARBY SPOTS | Show nearby spots | Results view - Checkbox in spot centric view |
Results Filtering And Sorting
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| LOCATION FILTER | Show spots in a selected area of the gel images | Results view - In the toolbar |
| PROFILE FILTER | Show spots that correspond to your chosen expression profile or trend | Results view - In the toolbar |
| FOLD CHANGE FILTER | Show spots with fold-change higher than your chosen value | Results view - In the toolbar |
| P-VALUE FILTER | Show only spots with p-value lower than your chosen value | Results view - In the toolbar |
| PRESENCE FILTER | Show only spots present in X% of the gel images | Results view - In the toolbar |
| VOLUME FILTER | Show only spots larger than a chosen value | Results view - In the toolbar |
| STAR RATING FILTER | Show only spots marked with a star | Results view - In the toolbar |
| SORT ON ANY FILTER | Sort spots according to any of the filters | Results view - In the toolbar |
| WRITE COMMENTS | Annotate comments for individual proteins | Results view - In spot centric view |
| PCA PLOT | Calculate and show a Principal Component Analysis plot | Results view - “PCA” icon in top right |
| EDIT SPOTS | Local spot editing: add, delete, move, split and merge spots | Results view - “Edit spots” button in spot centric view |
| EDIT MATCHING | Local match editing | Results view - “Edit spots” button in spot centric view |
| NORMALIZATION | Normalisation and background subtraction | Happens automatically |
Results Exporting
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| EXPORT TO PDF | Export PDF report | Results view - PDF icon in corner of results view |
| EXPORT TO XLS, CSV | Excel export of filtered results | Results view - Excel icon in corner of results view |
| EXPORT RAW DATA | Excel export of raw data | Start page - Export icon in the actions column |
| EXPORT GRAPHICS | Exporting of any images or graphs | Results view - Right click on any image or plot |
Picking
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| EXPORT TO GENERIC PICK FILE | Export generic pick file for picking robots | Results view - Picking icon in top right corner |
| EXPORT TO ETTAN | Export to Ettan picker | Results view - Picking icon in top right corner |
| EXPORT TO EXQUEST | Export to EXQuest picker | Results view - Picking icon in top right corner |
| EXPORT FOR MANUAL PICKING | Print gel image with spot IDs for manual picking | Results view - Picking icon in top right corner |
Payment And Account
| FEATURE | DESCRIPTION | WHERE TO FIND |
|---|---|---|
| REDFIN CREDITS | Use REDFIN credits to pay per gel | Start page - Account status in top right corner |
| PURCHASE CREDITS | Purchase REDFIN credits directly in the software | Start page - Click “buy credits” in top menu |
| VALUE CODE | Enter a value code in REDFIN to top up account with credits | Start page - Click “use value code” in top menu |
| CREDIT HISTORY | Log of credit purchase and usage | Start page - Click “my account in top menu |
| CREDIT STATUS | Current credit status | Start page - Credit status visable in top right corner |
| SECURITY | Change password option | Start page - Click “change password” in top menu |
