Solution Backgrounder

Ludesi has developed a unique, powerful solution to 2DGE image analysis that is made available as a service where users send gel images over the Internet to be analyzed by trained experts. Ludesi’s analysis software tools and unique protocol are designed to prevent error propagation and provide a guaranteed quality level and very high correctness in the image analysis.  

Analysis results at Ludesi typically show a large decrease in the number of false positives. This significantly reduces 'noise' in the analysis and frees the researcher from the burden of painstakingly trying to ascertain the validity of the results.  

Ludesi’s final analysis results will typically show a two- to threefold increase in the number of true hits (correct, statistically significant, changes in expression levels) compared to alternative 2DGE image analysis solutions.

In addition, the fact that the analysis is performed as a service by specialists frees the users from spending valuable time learning to use complicated software and performing the analysis themselves.

How the Ludesi 2DGE Image Analysis Solution Works

Generating the Analysis Results

Ludesi’s analysis of 2DGE images is performed as a globally accessible service.

First, the user uses the Ludesi REFIN Software to setup an experiment, then their experiment information, together with gel images, is sent to Ludesi over the Internet.

Next, the images are analyzed by professionals at the Ludesi Analysis Center. After analysis, the results are received by the scientist via download where the Ludesi REDFIN Software allows them to visualize and interpret the data.  

Ludesi has developed a comprehensive suite of technically advanced software analysis tools used only by the staff at Ludesi.

Unlike commercially available software products, Ludesi’s software does not compromise the analysis results by hiding any complexities or alternatives from the user.

The specialists at the Ludesi Analysis Center have extensive experience, specialized protocols, and expertise in performing these analyses which are the main reasons superior results are able to be achieved.

Getting the Analysis Results 

Utilizing a secure Web interface, the appliance-like, user-friendly Ludesi REDFIN Software enables scientists to work with the results of the analysis to visualize gel images, the spots, spot borders and matchings.

It also includes tools for performing a range of statistical analyses, cataloguing interesting proteins and classifying gels, as well as the ability to export all the raw data to work with using other tools if that is preferred.

End Results = Saving Significant Time, Training, and Costs

Because the Ludesi image analysis is performed as a service, it has enormous advantages in that the researcher can save significant time and effort by not having to learn or use analysis software. Instead, they can concentrate on the interpretation of the results.

The Ludesi service also eliminates the risk of bias introduced by a single scientist using analysis software without adhering to a strict protocol.

The result is better reproducibility and consistency independent from time and personal involvement.