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Clinical biomarker discovery: Dr. Angel Garcia successfully performs large-scale 2D-gel based proteomic analysis on clinical samples
Posted by ludesi in 2DE Knowledge Base, News
Ángel García Alonso (Principal Investigator, Ramón y Cajal Research Fellow), in the center; Andrés Fernández Parguiña (PhD student), to the left; and Isaac Rosa Benito (Lab Technician), to the right.
This weeks customer profile introduces you to Dr. Ángel García’s, who is using 2D gel based proteomics to research new antithrombotic drugs and novel platelet-related biomarkers in acute coronary syndromes.
Dr. García is a Ramón y Cajal Research Fellow at the Department of Pharmacology at the University of Santiago de Compostela in Spain. He has many years experience in platelet proteomics, most recently also at the Glycobiology Institute at the University of Oxford, UK. Dr. García is successfully performing large-scale 2D-gel based proteomic analysis on clinical samples.
Read below for Dr. García’s answers to our seven customer profile questions:
How would you describe the overall goal of your research?
(i) Identify novel signalling proteins that could play a key role in platelet activation and serve as potential drug targets as antithrombotics; (ii) identify novel platelet-related biomarkers in acute coronary syndromes.
What is your goal specifically for your 2D gel based research?
Efficient and sensitive differential analyses to achieve the above goals.
What type of samples are you primarily working with?
Primarily, platelets and plasma.
What have you achieved by using 2D gels?
We have dissected platelet signalling cascades and identified novel platelet proteins. Furthermore, we have demonstrated that platelets from patients with acute coronary syndrome express a characteristic proteomic profile.
What type of Ludesi analysis do you primarily use (REDFIN Solo, Basic, or Pro)?
Primarily, Pro and Solo.
What made you choose Ludesi’s analysis service?
The versatility, time-savings and good service (with quick response to feedback and implementation of new tools)
How does using the analysis service help you in your work?
The analysis helps me to achieve my goals quickly and with reliable results. I am very pleased with Ludesi and the way they get in touch whenever I have doubts. I hope they will carry on implementing new tools (e.g. nonparametric statistics, landmarking) that will further help my research and the reliability of the results.
Read more about Dr. García’s work in some of his recent publications:
JR. High-resolution two-dimensional gel electrophoresis analysis of atrial tissue proteome reveals down-regulation of fibulin-1 in atrial fibrillation.
García A, Eiras S, Parguiña AF, Alonso J, Rosa I, Salgado-Somoza A, Rico TY, Teijeira-Fernández E, González-Juanatey JR.
Int J Cardiol, in press.
Proteomic analysis of integrin alphaIIbbeta3 outside-in signaling reveals Src-kinase-independent phosphorylation of Dok-1 and Dok-3 leading to SHIP-1 interactions.
Senis YA, Antrobus R, Severin S, Parguiña AF, Rosa I, Zitzmann N, Watson SP, García A.
J Thromb Haemost. 2009 Oct;7(10):1718-26. Epub 2009 Aug 11.
Pubmed Link
A global proteomics approach identifies novel phosphorylated signalling proteins in GPVI-activated platelets: involvement of G6f, a novel platelet Grb2-binding membrane adapter.
García A, Senis YA, Antrobus R, Hughes CE, Dwek RA, Watson SP, Zitzmann N.
Proteomics. 2006 Oct;6(19):5332-43.
Pubmed Link
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