New in REDFIN: Wizard for adding and warping pick gel images tagged:

New in REDFIN: Wizard for adding and warping pick gel images

Posted by ludesi in New features in REDFIN

The picking icon in the REDFIN 2D gel image analysis software (as indicated by the red arrow)

In some experimental designs the gel you are picking spots from for protein identification will not be one of the gels used for image and data analysis. Mostly, this will be the case when the stain you are using is not compatible with, or optimal for, downstream mass spectrometry.

In those cases a designated “pick gel” is run.

This pick gel needs to be matched into the analysis project, so that interesting spot coordinates identified during image analysis can be transferred to the pick gel for subsequent spot excision.

But of course, you know all this.

What is important to you, is to have an easy way in the software to do it!

And that’s where we come in. With the new pick gel wizard in REDFIN we hope to have achieved just that.

Essentially, the pick gel wizard guides you through the following steps:

Step 1: tell REDFIN you would like to pick from a designated pick gel

Step 2: upload the pick gel image(s)

Step 3: Align the pick gel to one of the analytical gels via simplified image warping

Step 4: Export the picking coordinates for your spot cutting robot

Done!

And regardless if you did the image analysis yourself using Solo or requested a Basic or Pro analysis service, the adding and warping of pick gels is of course completely free.

We hope you enjoy using the picking wizard in REDFIN – we for one think it’s a pretty neat little addition to the software! :)