;)
How to add or remove gel images from an existing analysis
Posted by ludesi in How to's in REDFIN, Tips and Tricks
Here is a common scenario:
You analyze your 2D gel project, and start working with the results only to realize that you would actually like to change the comparison you have previously specified. Maybe there is a gel that didn’t run very well and is messing up your statistics? Or maybe your initial investigation has inspired you to create sub-groups and get the statistics on those? Is there a way that you can easily do that without re-analyzing the entire project?
The good news is that there is a very easy and fast way to do this in REDFIN.
Step 1: Open up an already analyzed project in REDFIN.
Step 2: Click on the “Manage comparisons” button in the top right corner of the screen
Step 3: Select the comparison you wish to edit or create a completely new comparison if you wish to keep the results of the existing analysis
Step 4: Select the group you wish to change or create a completely new group.
Step 5: Add or remove any images to the group by double clicking on it or by simply dragging and dropping it into the bottom image container.
Step 6: After you have made the required changes click on the “Back to results” button. All statistics are re-calculated on the fly.
With the Manage Comparison functionality you have the freedom to change and add comparisons as much as you wish, even after completing the initial analysis of your gels.
All comparisons you create are shown as different tabs enabling you to easily skip between them. The REDFIN results interface links all spot ID’s together, so that if you select one spot by clicking on it in the list, and you then click on a different comparison tab, the same spot ID is automatically shown. This allows you to quickly compare statistics on the same protein spot between different comparisons.
;)
