Latest Ludesi Pro customer success case: 2D gel study on birch pollen allergy gets published in JPR

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SEM image of pollen grains from a variety of plants

In the middle of the pollen season what fits better than reporting the latest news on the topic of pollen allergy from one of our customers.

The article published in the Journal of Proteome research and accessible in electronic version ahead of print, reports on the Human eosinophil proteome and changes induced by birch pollen allergy.

As a first step toward filling a missing link in understanding of the mechanisms of allergy, researchers from Uppsala University, Sweden, and International Centre for Genetic Engineering and Biotechnology, Trieste, Italy, aimed at identifying the human eosinophil proteome. Eosinophils are multifunctional white blood cells reported to control the mechanisms in allergic diseases.

Employing both 2D gel electrophoresis and 1D SDS-PAGE together with mass spectrometry the researchers could identify 336 spots/band corresponding to 98 different proteins.

Besides the global assessment of the eosinophil proteome, a comparative 2D gel image analysis was done to assess the changes of the proteome induced by birch pollen allergy.

Birch pollen allergic individuals and asymptomatic control individuals were bled during the peak of the birch pollen season and the isolated eosinophilic proteins were separated with 2D gel electrophoresis.

2D gel of birch pollen

2D gel with Eosinophil proteins marked that are differentially expressed during birch pollen allergy.

The resulting images were sent to Ludesi for a Pro analysis. With the help of the three dimensional profiling in REDFIN software the researchers could accurately excise and identify novel isoforms of a key protein that together with other observed changes gave novel information on pollen allergy induced changes to the proteome.

From key findings, the researchers concluded that the eosinophils in birch pollen allergic individuals outperform their counterpart cells in control individuals when it comes to motility and presenting the antigens.

Read more:

The Human Eosinophil Proteome. Changes Induced by Birch Pollen Allergy
Woschnagg C, Forsberg J, Engström A, Odreman F, Venge P, Garcia RC.
J Proteome Res. 2009 May 5. [Epub ahead of print]

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