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What settings should I use in order to optimize my image analysis results?
Posted by ludesi in 2DE Knowledge Base
Try to scan your gels in a high bit depth, preferably 16 bits. The images are still analyzable at 12 or 8 bits, but having 65,536 gray levels is more sensitive than having 256 gray levels for each pixel and therefore makes the analysis results more reliable. However, not all scanners are able to output 16 bit images.
A 16 bit file uses twice as much memory as an 8 bit file, but has 256 times as many gray levels. The extra accuracy provided by a 16 bit image is usually well worth the increase in file size.
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