;)
What is ”DPI” and ”bit depth”?
Posted by ludesi in 2DE Knowledge Base
DPI means ”dots per inch” and is a measure of resolution in your gel image file. Normal DPI values for 2D gel images range between 150 and 400 DPI, but this is highly dependent upon the size of the gel. The important thing is to make sure that the smallest spots are not too pixilated when you zoom in on them in the image. If you zoom in on the smallest spots in your gel and they still look acceptable you are probably fine.
Sometimes ”dots per centimeter” is used. 1 DPC is equal to 2.54 DPI.
The ”bit depth” is the number of binary bits containing information about the intensity value in each pixel in your gel image file. Common bit depths in grayscale images are 8 bits, 12 bits and 16 bits. Having 8 bits means a range of 2^8 = 256 possible values in your grey scale. Having 12 bits means 4,096 values, and 16 bits means 65,536 possible values in your image.
;)
